Probes hybridize with clone and fragment regions. Overlapping DNA fragments hybridize to same probe. 20-base oligonucleotides can be probes to find overlapping DNA fragments. For clone ends, random sequences can be probes {sequence-tagged connector} (STC). Probes can have minor-groove binder to enhance exact hybridization, allowing shorter probes.
Processing identifies unique 200-base to 500-base sequences, with unique primers, from known locations {sequence-tagged site} (STS). Perhaps, clones share STS.
4-Genetics-Recombinant DNA-DNA Sequencing
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Date Modified: 2022.0225